VNTR Analysis: The Science Behind DNA Fingerprinting

In this lab, students will analyze a single VNTR locus (Variable Number of Tandem Repeats) in several different subjects. The VNTR they will be studying, known as D1S80, is a 16 bp segment that is repeated 14 to 41 times in the genome. They will use agarose gel electrophoresis to separate the PCR products and compare the bands on the gel to a DNA ladder in order to determine each subject's genotype.

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